DON plate kit

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DON plate kit

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ELISA Plate Kit

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Product description

INTENDED USE

The Beacon Deoxynivalenol (DON) Plate Kit is a competitive ELISA for the quantitative analysis of vomitoxin in wheat, barley, malted barley, corn and oats.

ASSAY PRINCIPLES

The Beacon DON Plate Kit is a competitive enzyme-labeled immunoassay. DON is extracted from a ground sample by shaking with water. The aqueous extract is then filtered and the extract is tested in the immunoassay.DON-HRP enzyme conjugate is pipetted into the test wells followed by calibrators or sample extracts. DON antibody is then pipetted into the test wells to initiate the reaction. During the 10 minute incubation period, DON from the sample and DON-HRP enzyme conjugate compete for binding to DON antibody which, in turn, binds to the test well. Following this 10 minute incubation, the contents of the well are removed and the wells are washed to remove any unbound toxin or enzyme-labeled toxin. A clear substrate is then added to the wells and any bound enzyme-toxin conjugate causes the conversion to a blue color. Following a 5 minute incubation, the reaction is stopped and amount of color in each well is read. The color of unknown samples is compared to the color of the calibrators and the DON concentration of the samples is derived.

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